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D1-like dopamine receptor agonist. D1-like dopamine receptor agonist.
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A selective D1 dopamine receptor agonist.
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Image Search Results
Journal: bioRxiv
Article Title: Dopaminoceptive D1 and D2 neurons in ventral hippocampus arbitrate approach and avoidance in anxiety
doi: 10.1101/2023.07.25.550554
Figure Lengend Snippet: a , Experimental schematic. Male mice were injected in vSub with an AAV-dLight-1.1 (n = 15), an AAV-GRAB DA -1h (n = 15, ), an AAV-RdLight-1 (n = 6, ) or with a control AAV-GFP (n = 8) and implanted with optical fibers before recording during EPM testing. b , Representative traces during EPM exploration for a dLight-1.1 (top) and control GFP (bottom) animal. c , Spatially averaged fluorescence signal in the EPM for all dLight-1.1 (top-left) and all GFP (bottom-right) mice. d , Average dLight-1.1 signal during entries to (left) and exits from (right) the open arm (OA, blue) or closed arm as a control (CA, red). Only traces when the mouse did a complete closed arm to center (Ce) to open/closed arm are used for averaging. e , Average area under the curve (AUC) by EPM compartment for dLight-1.1 (left) and GFP (right) mice. LMM-ANOVA: compartment F 2,80 = 49.82 p < 0.0001, sensor F 3,40 = 1.70 p = 0.1822, compartment x sensor F 6,80 = 5.1529 p = 0.0002; followed by FDR-adjusted post-hoc tests. f , Experimental schematic for slice electrophysiological recordings from vCA1/vSub D1 or D2 pyramidal neurons identified in D1-Cre or D2-Cre x fl/fl eGFP::L10a male mice. g , Representative traces of resting membrane potential before, during and after bath application of dopamine (DA, 10 µM) or of the D1 agonist SKF81297 (SKF, 50 µM) onto D1 neurons (top), or of dopamine (DA, 10 µM) or of the D2 agonist quinpirole (Quin, 10 µM) onto D2 neurons (bottom). h , Quantification of resting membrane potential changes between baseline (BL), drug application and after wash (W). LMM-ANOVA: cell type F 1,5.8 = 0.90 p = 0.3805, agonist F 1,18.7 = 0.40 p = 0.5366, application period F 2,37.0 = 17.73 p < 0.0001, cell type x agonist F 1,18.7 = 0.51 p = 0.4851, cell type x application period F 2,37.0 = 16.39 p < 0.0001, agonist x application period F 2,37.0 = 0.23 p = 0.7972, cell type x agonist x application period F 2,37.0 = 0.02 p = 0.9821; followed by FDR-adjusted post-hoc tests. Data represented as mean ± sem.
Article Snippet: Dopamine hydrochloride (Tocris, #3548),
Techniques: Injection, Control, Fluorescence, Membrane